仔猪不同发育阶段结肠微生物菌群特征分析

doi:10.11843/j.issn.0366-6964.2019.09.004

摘要/Abstract

摘要： 旨在通过高通量测序技术比较马身猪与晋汾白猪不同生长发育阶段结肠微生物菌群结构与特征的差异。本研究以马身猪和晋汾白猪为试验对象，分别在仔猪1、28和70日龄3个阶段随机挑选体重相近的3头公猪，采用16S rRNA高通量测序技术对结肠微生物多样性进行研究。通过菌群分类学分析发现，两个品种仔猪肠道微生物分布于15个门、28个纲、59个目、100个科、290个属。厚壁菌门和拟杆菌门为优势菌门，平均所占比例分别为49.03%、31.94%。仔猪刚出生时品种间肠道微生物多样性差异不显著，随着仔猪生长发育，各品种微生物多样性出现极显著升高，在晋汾白猪仔猪保育阶段（28~70 d）基本趋于稳定。两品种仔猪不同阶段的特异菌群随着日龄增加出现显著差异。关联分析发现，肠道菌群与血清中内毒素（LPS）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）浓度之间存在显著相关性（P<0.05），而与二胺氧化酶（DAO）和D-乳酸（D-LA）浓度相关性不显著（P>0.05）。不同品种间，与LPS、TNF-α、IL-6显著相关的结肠微生物菌群存在差异。综上表明，仔猪结肠微生物菌群结构与组成在不同品种、不同生长发育阶段均存在显著差异，对仔猪免疫性能具有重要作用。

Abstract: The objective of this study was to compare the structure and composition of colon microflora of Mashen and Jinfen White pigs at different developmental stages using Illumina MiSeq sequencing technology. In present study, Mashen and Jinfen White pigs were used as experimental animals. Three boar piglets with similar weight were randomly selected at the stage of 1, 28 and 70 days old, respectively, and the colon microbial diversity was analyzed using 16S rRNA high-throughput sequencing technology. The intestinal microorganisms of piglets in two breeds were found to be distributed in 15 phyla, 28 classes, 59 orders, 100 families and 290 genus by bacteria taxonomy analysis. The Firmicutes and Bacteroides are the dominant bacteria, accounting for 49.03% and 31.94% respectively. There was no significant difference in intestinal microbial diversity of piglets at birth between breeds. The microbial diversity increased significantly with the growth and development of piglets, and basically stabilized at the stage of Jinfen White piglet conservation (28-70 d). The specific flora of two breeds of piglets at different stages showed significant difference with the increase of age. Association analysis showed that LPS, TNF-α, IL-6 were significantly correlated with intestinal flora (P<0.05), while the DAO and D-LA had no significant correlation with intestinal flora (P>0.05). The colonic microflora correlated with LPS,TNF-α,IL-6 was different between the two breeds. It is concluded that the structure and composition of colon microflora of piglets were significantly different between two breeds and among different developmental stages,which play the important roles in immune function of piglets.

中图分类号:

S811.6

郭晓红, 郭玉龙, 刘亚丹, 刘娟, 史文倩, 董磊, 蔡春波, 曹果清, 李步高, 高鹏飞. 仔猪不同发育阶段结肠微生物菌群特征分析[J]. 畜牧兽医学报, 2019, 50(9): 1759-1774.

GUO Xiaohong, GUO Yulong, LIU Yadan, LIU Juan, SHI Wenqian, DONG Lei, CAI Chunbo, CAO Guoqing, LI Bugao, GAO Pengfei. Characteristics of Intestinal Microflora of Colon at Different Developmental Stages in Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1759-1774.

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